So today's science lesson is about a technique called a Western blot that we often do in lab. The technique is used to test a cell culture for a certain protein by using a molecule (antibody) to attach to it and give off a fluorescent signal so you can see it. You run it down a charge gel to sort by size and then pour the antibody on it and take a picture. It's called a Southern blot because the original idea was from a guy named Edwin Southern probing for DNA. Then there's the Northern blot for RNA, Western for protein, Eastern for fatty acids, and over 10 more variations (South by Southwest, reverse Southern, far Eastern, etc...)....This is what one is supposed to look like....
This is what mine looked like.....
I'm not really sure what happened but needless to say I had to redo the experiment. I have deemed it to be "Modern Art" and have printed off and signed several copies for people on the floor. Better luck next time!
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