Tuesday, January 22, 2013

Pictures of cells

So here are some photos of my preliminary work in my lab. The first photo is a bright-field microscope shot of 293 T cells which are immune cells grown from embryonic kidney tissue that are good for transfection (infection) studies such as the one we are doing in the Wu lab.
 

The second photo is a fluorescence microscope photo of 293 T cells that were not infected with a virus. The virus contains a gene called GFP that produces a protein that glows green so you know when cells have been infected and you can thus measure the level of infectivity. Photo 2 is a negative control (was not infected) and thus it accurately shows no green proteins.
 

 This photo contains cells that have been infected and as you can readily see some of them are glowing green. After this we put the cells through a machine to see the infectivity rate and we go from there.
 I thought these photos were kind of cool looking and a good chance to give everyone a very basic idea of what I am doing here. Hope you enjoyed!








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